Tuesday, November 22, 2011

How does one know when a bacteria has taken in a cloning vector like a plasmid or phage?

I know how this occurs already, what with the restriction fragments, ligase, sticky ends and everything, but how do we know that the bacteria, say E. coli, have accepted the vector with the human gene inside?|||Usually, you also put a resistance gene or a gene along with your gene of interest that will only function if it had been taken up properly. So for instance, giving the bacteria an ampicillin resistance gene, and growing the bacteria on ampicillin containing medium, the ones that live will be the ones that accepted the vector succesfully.

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